关键词: 裸鼹鼠, 肾, 钙化, 慢性肾病, 动物模型

Abstract: Objective　To reveal the characteristics of spontaneous renal calcification during the development of naked mole rats, and to provide a valuable animal model for the study of renal calcification and prevention strategies for chronic kidney disease.Methods　Urine and serum were collected from Newborn, 12-month-old, and 120-month-old naked mole-rats for routine urinary and renal function tests; renal pathology was observed using HE staining, alizarin red staining, Von Kossa staining, and vWF staining; and metabolomics analysis was performed on kidney tissues of 12-month-old naked mole rats.Results　There were no significant differences in kidney function between 120-month old and 12-month-old NMRs. The vascular morphology was normal in NMRs with different ages and the calcification. Lipids and lipid-like substances, organic acids and their derivatives, undefined substances, organic heterocyclic compounds, and organic oxygen compounds accounted for 82.668% of the total metabolites in the kidneys of adult NMRs. Conclusion　By systematically exploring the functional characteristics of the kidneys of NMRs, especially the phenomenon of kidney calcification in NMRs as they age, it is revealed that spontaneous renal calcification of NMRs maintains a normal relationship with their kidney function.